Journal: Oncology Letters

Article Title: Analysis of the clonal origin and differences in the biological behavior of multifocal papillary thyroid carcinoma

doi: 10.3892/ol.2024.14677

Figure Lengend Snippet: Histology of individual multifocal papillary thyroid carcinoma samples. Representative images of (A) BRAF protein-positive, (B) BRAF protein-negative, (C) TERT protein-positive and (D) TERT protein-negative samples. Magnification, ×40. BRAF, v-raf murine sarcoma viral oncogene homolog B1; TERT, telomerase reverse transcriptase.

Article Snippet: The primary antibodies used were mouse anti-human BRAF monoclonal antibody (cat no. TA500431) and rabbit anti-human TERT polyclonal antibody (cat. no. TA324097), both from OriGene Technologies, Inc, a dilution of 1:200.

Techniques: Reverse Transcription

Journal: Oncology Letters

Article Title: Analysis of the clonal origin and differences in the biological behavior of multifocal papillary thyroid carcinoma

doi: 10.3892/ol.2024.14677

Figure Lengend Snippet: BRAF V600E mutation in multifocal papillary thyroid carcinoma. Representative (A) BRAF V600E mutant and (B) wild-type amplification plots generated using quantitative PCR. The red marking represents the threshold line. BRAF, v-raf murine sarcoma viral oncogene homolog B1; BRAF-IC, BRAF internal reference gene detection curve; BRAF-W, BRAF V600E wild-type gene detection curve; BRAF-M, BRAF V600E mutation gene detection curve; DRn, change in relative fluorescence normalized.

Article Snippet: The primary antibodies used were mouse anti-human BRAF monoclonal antibody (cat no. TA500431) and rabbit anti-human TERT polyclonal antibody (cat. no. TA324097), both from OriGene Technologies, Inc, a dilution of 1:200.

Techniques: Mutagenesis, Amplification, Generated, Real-time Polymerase Chain Reaction, Fluorescence

Journal: Oncology Letters

Article Title: Analysis of the clonal origin and differences in the biological behavior of multifocal papillary thyroid carcinoma

doi: 10.3892/ol.2024.14677

Figure Lengend Snippet: Gene mutation and protein expression in cases of multifocal papillary thyroid carcinoma with different clonal origins.

Article Snippet: The primary antibodies used were mouse anti-human BRAF monoclonal antibody (cat no. TA500431) and rabbit anti-human TERT polyclonal antibody (cat. no. TA324097), both from OriGene Technologies, Inc, a dilution of 1:200.

Techniques: Mutagenesis, Expressing

Journal: Brain Pathology

Article Title: New clinicopathological associations and histoprognostic markers in ILAE types of hippocampal sclerosis

doi: 10.1111/bpa.12596

Figure Lengend Snippet: CD34+ cells are associated with rarer patterns of hippocampal sclerosis. A–D, I–J. A CD34+ nodular case. CD34 immunopositive/CD34+ stellate cells formed a nodule (solid arrowheads in (A‐B). Nestin immunolabeling showed a frequent extravascular stellate pattern in the area of the CD34+ nodule (solid arrowhead in C) whereas Vimentin immunolabeling showed no extravascular immunolabeling (D). E–H, K–L. A CD34+ scarce case. E. An isolated CD34+ extravascular stellate cell is observed (open arrowheads in E, F). Nestin immunolabeling showed a scant stellate extravascular pattern in the area of the CD34+ stellate cell (open arrowhead in G) whereas Vimentin immunolabeling showed no extravascular immunolabeling (H). Double immunolabelings showed the co‐expression in stellate cells of Nestin (brown) and CD34 (red) (I), and SOX2 (pink) and CD34 (brown) (J) in a CD34+ nodular case. Double immunolabelings showed the co‐expression in stellate cells of Nestin (brown) and CD34 (red) (K, higher magnification in insert), and of SOX2 (pink) and CD34 (brown) (L) in a CD34+ scarce case. M–P. Distribution of CD34 immunolabeling (CD34 negative in black, CD34+ scarce in orange and CD34+ nodular in green) for type 1 (M), type 2 (N), type 3 (O), the pattern noHS (P). Q–S. A CD34+ nodular case with BRAF V600E immunopositive neurons. Q. A moderate immunolabeling of scarce cells by P53 (solid arrowheads). The BRAF V600E immunolabeling is positive in neurons (solid arrowheads in r,s). T. A CD34+ scarce case with BRAF V600E+ neurons (open arrowhead). Scale bars: (A,E) 400 μm; (B–D,F–H) 100 μm; (I–L,T) 50μm; (Q,R) 200μm; (S) 20μm.

Article Snippet: Primary antibodies were as follows: BRAF V600E (mouse monoclonal VE1, 1:100, Abcys Eurobio), CD31 (monoclonal mouse JC70A, 1:30, Dako) CD34 (monoclonal mouse QBEnd10, 1:50, Dako), ELAVL2 (rabbit polyclonal, 1:500, Proteintech), GFAP (monoclonal mouse 6F2, 1:500, Dako), Internexin alpha/INA (monoclonal mouse 2E3, 1:100, Quartett Biochemicals), Nestin (monoclonal mouse 10C2, 1:2000, Sigma‐Aldrich), NeuN (Monoclonal mouse MAB377, 1:100; Merck Millipore), OLIG2 (rabbit monoclonal EP112, 1:200, Epitomics), P53 (monoclonal mouse, DO‐7, 1:100 Dako), SOX2 (rabbit monoclonal SP76, 1:300, Sigma‐Aldrich), Vimentin (monoclonal mouse V9, 1:50 Dako).

Techniques: Immunolabeling, Isolation, Expressing

Journal: Brain Pathology

Article Title: New clinicopathological associations and histoprognostic markers in ILAE types of hippocampal sclerosis

doi: 10.1111/bpa.12596

Figure Lengend Snippet: Classification of CD34+ hippocampal sclerosis according to BRAF V600E and INA status. A,C,E,G–I. A case of “BRAF V600E+ HS” with BRAF V600E positive neurons. B,D,F,J–L. A case of hippocampal MVNT. A,B. CD34 immunolabeling showed a nodular pattern of CD34 positive stellate cells (solid arrowheads). C,D. NeuN immunolabeling showed a neuronal loss that is predominant in CA4 (solid arrowheads) corresponding to HS ILAE type 3. E. Absence of INA positive abnormal neurons. F. Presence of numerous abnormal INA+ neurons (solid arrowheads). G–I. Absence of abnormal neurons according to NeuN, ELAVL2 and INA immunolabelings in the white matter of CA2 (open arrowheads) in a BRAF V600E+ HS. J–L. Presence of Neun Negative, ELAVL2 positive, INA positive abnormal neurons (solid arrowheads) in the white matter of CA2 in a MVNT. Scale bars: (A–F) 2.5 mm; (G–L) 200 μm. Abbreviations: INA = internexin alpha; MVNT = multinodular and vacuolating neuronal tumor.

Article Snippet: Primary antibodies were as follows: BRAF V600E (mouse monoclonal VE1, 1:100, Abcys Eurobio), CD31 (monoclonal mouse JC70A, 1:30, Dako) CD34 (monoclonal mouse QBEnd10, 1:50, Dako), ELAVL2 (rabbit polyclonal, 1:500, Proteintech), GFAP (monoclonal mouse 6F2, 1:500, Dako), Internexin alpha/INA (monoclonal mouse 2E3, 1:100, Quartett Biochemicals), Nestin (monoclonal mouse 10C2, 1:2000, Sigma‐Aldrich), NeuN (Monoclonal mouse MAB377, 1:100; Merck Millipore), OLIG2 (rabbit monoclonal EP112, 1:200, Epitomics), P53 (monoclonal mouse, DO‐7, 1:100 Dako), SOX2 (rabbit monoclonal SP76, 1:300, Sigma‐Aldrich), Vimentin (monoclonal mouse V9, 1:50 Dako).

Techniques: Immunolabeling

Journal: Brain Pathology

Article Title: New clinicopathological associations and histoprognostic markers in ILAE types of hippocampal sclerosis

doi: 10.1111/bpa.12596

Figure Lengend Snippet: Radiological features of BRAF V600E+ hippocampal sclerosis and hippocampal MVNT. A‐B. BRAF V600E+ hippocampal sclerosis. Coronal T2 sequence showing discrete atrophy, loss of digitation and T2 hyperintensity of the right hippocampus (vertical arrows). C–E. Hippocampal MVNT. Coronal T2 (C), FLAIR (D) and T1 (E) sequences show discrete atrophy of left internal temporal structures (vertical arrows), associated with extensive T2 and FLAIR hyperintensities involving the hippocampus, parahippocampal gyrus, fusiform gyrus and part of the inferior temporal gyrus (oblique arrows). A pseudocyst (arrowheads) is visible within the fusiform gyrus, hyperintense on T2 and hypointense on T1 and FLAIR images.

Article Snippet: Primary antibodies were as follows: BRAF V600E (mouse monoclonal VE1, 1:100, Abcys Eurobio), CD31 (monoclonal mouse JC70A, 1:30, Dako) CD34 (monoclonal mouse QBEnd10, 1:50, Dako), ELAVL2 (rabbit polyclonal, 1:500, Proteintech), GFAP (monoclonal mouse 6F2, 1:500, Dako), Internexin alpha/INA (monoclonal mouse 2E3, 1:100, Quartett Biochemicals), Nestin (monoclonal mouse 10C2, 1:2000, Sigma‐Aldrich), NeuN (Monoclonal mouse MAB377, 1:100; Merck Millipore), OLIG2 (rabbit monoclonal EP112, 1:200, Epitomics), P53 (monoclonal mouse, DO‐7, 1:100 Dako), SOX2 (rabbit monoclonal SP76, 1:300, Sigma‐Aldrich), Vimentin (monoclonal mouse V9, 1:50 Dako).

Techniques: Sequencing